Description

The antibody has a provenstrong Biotin-Streptavidin/HRP immunostaining at a 1/500-1/1000 dilution in rat hypothalamus and spinal cord. The specificity of the antiserum was examined by soluble pre-adsorption with the peptides in question at a final concentration of 10-6M CCK-8 immunolabeling was completely abolished by pre-adsorption with CCK-8, gastrin 17 and gastrin 34. Pre-adsorption with the following peptides resulted in no reduction of immunostaining: α-CGRP, Ÿ-CGRP, neurotensin, somatostatin, substance P, leucine enkephalin, methionine enkephalin, VIP, neuropeptide Y, gastric inhibitory polypeptide, bombesin, glucagon, peptide YY, and FMRF amide.

Product Description: Low magnification IHC image of neurons staining for CCK-8 in the dorsal horn of the rat spinal cord (above), and in the rat hippocampus (below). The tissue was fixed with 4% formaldehyde in phosphate buffer, before being removed and prepared for vibratome sectioning (hippocampus) and frozen sectioning (spinal cord). Sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:1000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen.

Host: Rabbit

Quantity / Volume: 100 µL

State: Lyophilized Whole Serum

Reacts With: Blowfly, Bubalus Bubalis (Water Buffalo), Cat, Chick, Decapod Crustacea, Gerbil, Guinea Pig, Hamster, Human, Lizard, Lobster, Mollusk, Mollusk, Monkey, Mouse, Mudpuppy (Necturus Maculosus), Pigeon, Rabbit, Raccoon, Rat, Sheep, Snail, Snail (Bulla Gouldiana) , Sting Ray, Tree Frog, Trout, Zacco Platypus (Minnow)

Availability: In Stock

Preabsorption Control: Available at Sigma Aldrich, Catalog # C2901

Alternate Names: Cholecystokinin-8; CCK, anti-CCK

RRID: AB_572224Immunogen: Sulfated CCK-8 (26-33)Gene Symbol / ID, Accession #: CCK,885